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Advancing Cell Therapy: Photothermal nanofibers enable macromolecule delivery in unstimulated human T cells

In the pursuit of refining adoptive T cell transfer, a critical facet of cell therapies, a recent study Ghent University delves into the application of Photothermal Electrospun Nanofibers (PEN) for intracellular delivery. This technique has proven efficient for activated T cells and now presents a promising avenue for unstimulated T cells.

Key Findings: A Glimpse into the Future of T-Cell Modification.

Safety Assurance: Inductively coupled plasma tandem mass spectrometry confirms no significant iron release from PEN substrates at laser fluences up to 0.36 J/cm².

High Delivery Efficiency: Successful intracellular delivery of 150 kDa FITC-dextran attains a notable 55–60% efficiency in resting and pre-activated lymphocytes.

Minimal Impact on T Cell Properties: Rigorous analysis indicates no discernible effects on cell proliferation, activation propensity, and extracellular cytokine release.

Towards Genetic Modification: With these compelling outcomes, PEN photoporation emerges as a secure and efficient delivery strategy, setting the stage for its role in the genetic modification of minimally differentiated T cells.

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